COPD/lung emphysema

COPD/lung emphysema

The expertise of CURAX in measuring lung function in vivo is also used to study the pathology of COPD. Most of these experiments are performed in mice. However, CURAX also has expertise on in vitro experiments using cigarette smoke as an activating agent.

In vitro models:
Cigarette smoke-induced cytokine and chemokine production by human monocytes, neutrophils, mast cells and dendritic cells.

COPD animal models:
Cigarette smoke induced lung emphysema
Endotoxine-induced lung emphysema
Collagen-induced lung emphysema

Outcome:
Heart hypertrophy
Airway function
Airway responsiveness in vitro and in vivo
Long emphysema, long morphology
Long volume
Pre-collagen formation
Elastase activity
Airway inflammation
Cytokines & inflammatory mediators
Oxidative stress

Literature:

1) Givi et al., Cigarette smoke differentially modulates dendritic cell maturation and function in time. Respir Res. 2015 24;16:131.

2) Abdul Roda et al., Targeting prolyl endopeptidase with valproic acid as a potential modulator of neutrophilic inflammation. PLoS One. 2014;9:e97594. 

3) Mortaz et al., A relation between TGF-β and mast cell tryptase in experimental emphysema models. Biochim BiophysActa. 2012;1822(7):1154-60

4) Givi et al., Dendritic cells inversely regulate airway inflammation in cigarette smoke-exposed mice. Am J Physiol Lung Cell Mol Physiol. 2016 1;310(1):L95-L102.